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anti-cd135 (clone a2f10)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti-cd135 (clone a2f10)

    Anti Cd135 (Clone A2f10), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-cd135+(clone+a2f10)/cd135+a2f10+antibody/pmc10772324-26-0-4
    Average 90 stars, based on 1 article reviews
    anti-cd135 (clone a2f10) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis"

    Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis

    Journal: Cell Reports Medicine

    doi: 10.1016/j.xcrm.2023.101256


    Figure Legend Snippet:

    Techniques Used: Control, Recombinant, Staining, Cell Isolation, Software

    Related Articles

    other:

    Article Title: In situ maturation and tissue adaptation of type 2 innate lymphoid cell progenitors
    Article Snippet: Anti-mouse Flt3/CD135 PerCP–eFluor 710 (Clone A2F10) , eBioscience , Cat46-1351-80.

    Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis
    Article Snippet: Anti CD135 (clone A2F10) , eBioscience , Cat# 15-1351-82; RRID: AB_494219.

    Article Title: CLEC11a is a bone growth agent
    Article Snippet: The following antibodies were used to identify restricted hematopoietic progenitors: anti-CD34-FITC (eBioscience, clone RAM34, 1:100); anti-CD16/32-Alexa Fluor 700 (eBioscience, clone 93, 1:200); anti-CD135-PEcy5 (eBioscience, clone A2F10, 1:100); anti-CD127-Biotin (BioLegend, clone A7R34, 1:200)+Streptavidin-PE-CF592 (BD Biosciences, 1:500); anti-cKit-APC-eFluor780 (eBioscience, clone 2B8, 1:200); anti-ScaI-PEcy7 (eBioscience, clone E13-161.7, 1:200) and lineage markers listed above.

    Article Title: Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosis.
    Article Snippet: For flow cytometry, 1x106 cells were added in a 96-well V-bottom plate (Thermo Fisher Scientific) and incubated with 2.5 mg/ml of a blocking anti-CD16/32 antibody (Clone 90, Thermo Fisher Scientific, eBioscience) diluted in 1XDPBS supplementedwith 1%FCS for 20 min at 4 C. After two washing steps, cells were stained with the following monoclonal antibodies: anti-CD11b APC (clone M1/70, Thermo Fisher Scientific, eBioscience), anti-Ly6C FITC (clone HK1.4, BioLegend), anti-Ly6G PE (clone 1A8, BioLegend), anti-F4/80 PerCP-Cy5.5 (clone BM8, BioLegend), anti-C5aR1 (CD88) PE-Cy7 (clone 20/70, BioLegend), anti-C5aR2 (C5L2) Alexa Fluor 700 (clone 468705, R&D Systems, Minneapolis, MN, USA), anti-C3aR Alexa Fluor 488 (14D4, Hycult Biotech, Wayne, PA, USA), antiC3/C3b/iC3b,/C3c Alexa Fluor 488 (clone 3/26, Hycult), anti-C3a Alexa Fluor 488 (clone mAb 3/11, Hycult), anti-CD16/32 APC (clone 93, Thermo Fisher Scientific, eBioscience), anti-TIM4 PE (clone 54 (RM14-54), Thermo Fisher Scientific, eBioscience) anti-MHCII Immunity 56, 1809–1824.e1–e10, August 8, 2023 e5 Alexa Fluor 700 (cloneM5/114.15.2, Thermo Fisher Scientific, eBioscience), anti-VSIG4 PE-Cy7 (clone NLA14, Thermo Fisher Scientific, eBioscience), FITC-labelled hematopoietic lineage cocktail (Thermo Fisher Scientific, eBioscience), anti-c-kit (CD117) APCeFluor 780 (clone 2B8, Thermo Fisher Scientific, eBioscience), anti-Sca1 (Ly6A/E) PE-Cy7 (clone J43, Thermo Fisher Scientific, eBioscience), anti-CD34 eFluor 450 (clone RAM34, Thermo Fisher Scientific, eBioscience), anti-CD135 (Flt3) PerCP-eFluor 710 (clone A2F10, Thermo Fisher Scientific, eBioscience) and anti-IL7R (CD127) PE (clone A7R34, Thermo Fisher Scientific, eBioscience).

    Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis
    Article Snippet: Anti-CD135 (clone A2F10) , eBioscience , Cat# 13-1351-85; RRID: AB_466600.

    Article Title: Clonal lineage tracing reveals shared origin of conventional and plasmacytoid dendritic cells.
    Article Snippet: Article Clonal lineage tracing reve als shared origin of conventional and plasmacytoid dendritic cells

    Flow Cytometry:

    Article Title: Expression of a constitutively active p38α mutant in mice causes early death, anemia, and accumulation of immunosuppressive cells.
    Article Snippet: 1 CREATE-NUS-HUJ, Molecular Mechanisms of Inflammatory Diseases Program, National University of Singapore, Singapore 2 Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 3 Department of Pharmacology, Yong Loo Lin School of Medicine, National University Health System, Singapore 4 Department of Biological Chemistry, The Institute of Life Science, The Hebrew University of Jerusalem, Israel 5 Animal Gene Editing Laboratory (AGEL), Biological Resource Centre, Agency for Science, Technology and Research (A*STAR), Singapore 6 Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore 7 Cancer Science Institute of Singapore, National University of Singapore, Singapore 8 Immunology Programme, Life Science Institute, National University of Singapore, Singapore

    Staining:

    Article Title: Hypomorphic Brca2 and Rad51c double mutant mice display Fanconi anemia, cancer and polygenic replication stress
    Article Snippet: .. The following antibodies were used to stain for hematopoietic stem cells (HSCs) and progenitor populations: biotin-conjugated lineage cocktail with antibodies anti-TER-119, anti-CD11b, anti-Ly-6G/Ly-6C (Gr-1), anti-CD3e and anti-CD45R/B220 (BioLegend), anti-streptavidin (APC-Cy7, BD Pharmingen) was used as the secondary antibody, anti-c-kit (APC, clone 2B8, BD Pharmingen), anti-Sca-1 (PerCP-Cy5.5, clone D7, eBioscience), anti-CD34 (FITC, clone RAM34, eBioscience), anti-CD135 (Flt3) (PE, clone A2F10, eBioscience), and anti-CD127 (PE-Cy7, clone SB/199, eBioscience). ..



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    (A) FACS-gal analysis of TCRβ + (αβ T cells, TCRγδ T cells and B220 + B cells in the spleen. As a positive control, LacZ Tg TCRβ + splenocytes were also analyzed. (B) Analysis of total thymocytes. (C) Analysis of total bone marrow (BM) cells, Lineage - Sca1 + cKit + (LSK) gated BM cells, which containing the hematopoietic stem cells, common myeloid progenitor (GMP) identified as Lineage - Sca1 - cKit + CD34 + CD16/32 lo or common lymphoid progenitor (CLP) identified as Lineage - Sca1 + cKit int <t>CD127</t> + CD135 + . A representative of five independent experiments is shown.
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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis

    doi: 10.1016/j.xcrm.2023.101256

    Figure Lengend Snippet:

    Article Snippet: Anti-CD135 (clone A2F10) , eBioscience , Cat# 13-1351-85; RRID: AB_466600.

    Techniques: Control, Recombinant, Staining, Cell Isolation, Software

    Journal: Cell Reports Medicine

    Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis

    doi: 10.1016/j.xcrm.2023.101256

    Figure Lengend Snippet:

    Article Snippet: Anti CD135 (clone A2F10) , eBioscience , Cat# 15-1351-82; RRID: AB_494219.

    Techniques: Control, Recombinant, Staining, Cell Isolation, Software

    Journal: iScience

    Article Title: BATF2 promotes HSC myeloid differentiation by amplifying IFN response mediators during chronic infection

    doi: 10.1016/j.isci.2023.106059

    Figure Lengend Snippet:

    Article Snippet: Anti-Mouse Flk2/Flt3/CD135 (PE conjugated, clone A2F10) , ebioscience , Cat#12-1351-82, RRID: AB_465859.

    Techniques: Sequencing, Blocking Assay, Recombinant, SYBR Green Assay, Western Blot, Mass Spectrometry, Software

    (A) FACS-gal analysis of TCRβ + (αβ T cells, TCRγδ T cells and B220 + B cells in the spleen. As a positive control, LacZ Tg TCRβ + splenocytes were also analyzed. (B) Analysis of total thymocytes. (C) Analysis of total bone marrow (BM) cells, Lineage - Sca1 + cKit + (LSK) gated BM cells, which containing the hematopoietic stem cells, common myeloid progenitor (GMP) identified as Lineage - Sca1 - cKit + CD34 + CD16/32 lo or common lymphoid progenitor (CLP) identified as Lineage - Sca1 + cKit int CD127 + CD135 + . A representative of five independent experiments is shown.

    Journal: PLoS ONE

    Article Title: MYL9 deficiency is neonatal lethal in mice due to abnormalities in the lung and the muscularis propria of the bladder and intestine

    doi: 10.1371/journal.pone.0270820

    Figure Lengend Snippet: (A) FACS-gal analysis of TCRβ + (αβ T cells, TCRγδ T cells and B220 + B cells in the spleen. As a positive control, LacZ Tg TCRβ + splenocytes were also analyzed. (B) Analysis of total thymocytes. (C) Analysis of total bone marrow (BM) cells, Lineage - Sca1 + cKit + (LSK) gated BM cells, which containing the hematopoietic stem cells, common myeloid progenitor (GMP) identified as Lineage - Sca1 - cKit + CD34 + CD16/32 lo or common lymphoid progenitor (CLP) identified as Lineage - Sca1 + cKit int CD127 + CD135 + . A representative of five independent experiments is shown.

    Article Snippet: The following anti-mouse antibodies (all from Thermo Fisher Scientific, Waltham) were employed: TCRβ (clone H57-597), TCRγδ (clone eBioGL3), B220 (clone RA3-6B2), Sca1 (clone D7), cKit (clone 2B8), CD34 (clone RAM34), CD16/32 (clone 93), CD127 (clone eBioSB/199) and CD135 (clone A2F10).

    Techniques: Positive Control

    Journal: Cell reports

    Article Title: Mds1 CreERT2 , an inducible Cre allele specific to adult-repopulating hematopoietic stem cells

    doi: 10.1016/j.celrep.2021.109562

    Figure Lengend Snippet:

    Article Snippet: Rat anti-Mouse CD135 (Flt3) PE, Clone: A2F10 , eBioscience , Cat# 50–106-20.

    Techniques: Virus, Recombinant, One Step RT-PCR, Software