anti-cd135 (clone a2f10) (Thermo Fisher)
Structured Review

Anti Cd135 (Clone A2f10), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-cd135+(clone+a2f10)/cd135+a2f10+antibody/pmc10772324-26-0-4
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis"
Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis
Journal: Cell Reports Medicine
doi: 10.1016/j.xcrm.2023.101256
Figure Legend Snippet:
Techniques Used: Control, Recombinant, Staining, Cell Isolation, Software
Related Articles
other:Article Title: In situ maturation and tissue adaptation of type 2 innate lymphoid cell progenitors Article Snippet: Anti-mouse Flt3/CD135 PerCP–eFluor 710 (Clone A2F10) , Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis Article Snippet: Article Title: CLEC11a is a bone growth agent Article Snippet: The following antibodies were used to identify Article Title: Cell-autonomous regulation of complement C3 by factor H limits macrophage efferocytosis and exacerbates atherosclerosis. Article Snippet: For flow cytometry, 1x106 cells were added in a 96-well V-bottom plate (Thermo Fisher Scientific) and incubated with 2.5 mg/ml of a blocking anti-CD16/32 antibody (Clone 90, Thermo Fisher Scientific, eBioscience) diluted in 1XDPBS supplementedwith 1%FCS for 20 min at 4 C. After two washing steps, cells were stained with the following monoclonal antibodies: anti-CD11b APC (clone M1/70, Thermo Fisher Scientific, eBioscience), anti-Ly6C FITC (clone HK1.4, BioLegend), anti-Ly6G PE (clone 1A8, BioLegend), anti-F4/80 PerCP-Cy5.5 (clone BM8, BioLegend), anti-C5aR1 (CD88) PE-Cy7 (clone 20/70, BioLegend), anti-C5aR2 (C5L2) Alexa Fluor 700 (clone 468705, R&D Systems, Minneapolis, MN, USA), anti-C3aR Alexa Fluor 488 (14D4, Hycult Biotech, Wayne, PA, USA), antiC3/C3b/iC3b,/C3c Alexa Fluor 488 (clone 3/26, Hycult), anti-C3a Alexa Fluor 488 (clone mAb 3/11, Hycult), anti-CD16/32 APC (clone 93, Thermo Fisher Scientific, eBioscience), Article Title: FLT3L-dependent dendritic cells control tumor immunity by modulating Treg and NK cell homeostasis Article Snippet: Article Title: Clonal lineage tracing reveals shared origin of conventional and plasmacytoid dendritic cells. Article Snippet: Article Clonal lineage tracing reve als shared origin of conventional and plasmacytoid dendritic cells Flow Cytometry:Article Title: Expression of a constitutively active p38α mutant in mice causes early death, anemia, and accumulation of immunosuppressive cells. Article Snippet: 1 CREATE-NUS-HUJ, Molecular Mechanisms of Inflammatory Diseases Program, National University of Singapore, Singapore 2 Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 3 Department of Pharmacology, Yong Loo Lin School of Medicine, National University Health System, Singapore 4 Department of Biological Chemistry, The Institute of Life Science, The Hebrew University of Jerusalem, Israel 5 Animal Gene Editing Laboratory (AGEL), Biological Resource Centre, Agency for Science, Technology and Research (A*STAR), Singapore 6 Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore 7 Cancer Science Institute of Singapore, National University of Singapore, Singapore 8 Immunology Programme, Life Science Institute, National University of Singapore, Singapore Staining:Article Title: Hypomorphic Brca2 and Rad51c double mutant mice display Fanconi anemia, cancer and polygenic replication stress Article Snippet: .. The following antibodies were used to stain for hematopoietic stem cells (HSCs) and progenitor populations: biotin-conjugated lineage cocktail with antibodies anti-TER-119, anti-CD11b, anti-Ly-6G/Ly-6C (Gr-1), anti-CD3e and anti-CD45R/B220 (BioLegend), anti-streptavidin (APC-Cy7, BD Pharmingen) was used as the secondary antibody, anti-c-kit (APC, clone 2B8, BD Pharmingen), anti-Sca-1 (PerCP-Cy5.5, clone D7, eBioscience), anti-CD34 (FITC, clone RAM34, eBioscience), |


